You expect a lot from your real-time qPCR reagents—they need to perform at a high level and provide reproducibility, instrument compatibility, and sensitivity at a great value. Our real-time qPCR kits and master mixes are designed with these issues in mind. Whether your experiments are routine, complicated, or proving difficult, we have the right qPCR tools to move your research forward.

RNA-Seq utilizes high-throughput sequencing technology to detect and measure the transcriptional readout (transcriptome) in a biological system. Analysis of RNA-Seq data allows expression quantification of different ribonucleic acids (RNAs) including mRNAs, miRNAs and lncRNAs. This data can be used to determine the differential gene expression patterns between different conditions, such as healthy vs disease. In addition, RNA-Seq can also be used to study RNAs structure or post-transcriptional splicing and predict its regulation mechanism in various conditions like complex diseases.

DNA sequence motif, a short sequence pattern which carried important functional properties in the genome which one of the most well known is protein specific binding in cis-regulation of gene expression. By analysing the regulatory sequence like promoter sequence upstream of a gene set, we can profile the footprint of regulatory protein(s) like transcription factors that involve in the expression regulation. This analysis can be performed with data from different assays such as:

• Sequences containing mutation or SNP from resequencing data
• Differentially expressed genes identified in RNA-Seq or expression arrays
• Chromatin regions with differential accessibility signal identified in ATAC-Seq
• Chromatin regions with histone modification signal from ChIP-Seq