COVID-19 Coronavirus

Real Time PCR Kit

The COVID-19 Coronavirus Real Time PCR Kit is a real-time
fluorescent RT-PCR based assay intended for the qualitative
detection of SARS-CoV-2 nucleic acid in nasopharyngeal
swabs and oropharyngeal (throat) swabs from individuals who are
suspected of COVID-19 by their healthcare provider.

SARS-CoV-2 RT-PCR Detection Kit

Negative Control

Positive Control

Reaction Mix

Morzon SARS-CoV -2 qPCR Kit is a real-time RT-PCR test intended for the qualitative detection of RdRp and N gene of SARS-CoV-2 RNA in single tube in which human RNase P is simultaneously detected.
Morzon SARS-CoV-2 qPCR Kit is a one-step real time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to produce complementary DNA (cDNA) strands and subsequently DNA polymerase-mediated cDNA amplification occurs. During cDNA replication in the PCR process, the hydrolysis probe hybridizes to the template and is digested by the 5′-3′ endonuclease activity of the Thermus aquaticus (Taq)DNA polymerase as the PCR primer is extended. The probe is digested only if cDNA replication occurs, separating the fluorescent and quencher molecules. The PCR products are detected within minutes by monitoring the increase in fluorescence that occurs exponentially with successive PCR amplification cycles. The method is performed directly on RNA extracted from the patient specimens. The detection of SARS-CoV-2 RNA is done in single tube in which human RNase P is simultaneously detected. COVID-19 real-time PCR assay utilizes human RNase P as an internal control, which controls for target isolation and amplification. The oligonucleotide primers and probes for detection of SARS-CoV-2 were selected from regions of the virus conserved region of RdRp and N gene. The kit is designed for specific detection of the SARS-CoV-2 (two primer/probe sets).

Product Features

  •  Double-target detection of genes to improve diagnosis accuracy
  • The RdRp and N gene of SARS-Cov-2 will be detected by FAM channel, and the RNase P gene of internal control will be detected by HEX channel.
  •  Negative and positive controls provide calibration for the kit
  • The detection of SARS-CoV-2 RNA is done in single tube in which human RNase P is simultaneously detected.
  •  With great clinical performance
  • Clinical evaluation study was performed to evaluate the performance of the SARS-Cov-2RT-PCR Detection Kit. Compared with the comparator method, the PPA was 99.07%, andthe NPA was 98.97%.

Steps for Simple Test


NOTE: Test results are for the identification of SARS-CoV-2 RNA. The SARS-CoV-2 RNA is generally detectable respiratory specimens during the acute phase of infection. Positive results are indicative of the presence of SARS-CoV-2 RNA. Clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of disease. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information.